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Isolation and Identification of Penicillium toxicarium: A New Record for Turkey

Year 2022, Volume: 13 Issue: 1, 44 - 54, 28.04.2022

Safiye Elif Korcan Tuğba Kahraman İjlal Ocak Ahmed Badri Abed Büşra Aydın

Abstract

In this study Penicillium toxicarium was cited for the first time as a new record of Penicillium in Turkey. This species was isolated from decaying wood samples in Usak province, Turkey. Morphological, colonial and molecular identification were performed. Fungal DNA Isolation Kit and universal internal transcribed spacer (ITS) primers were used for molecular identification.

Keywords

Penicillium toxicarium Penicillium Turkey Fungi Mycobiota Record

References

  • Abastabar, M., Mirhendi, H., Hedayati, M.T., Shokohi, T., Matehkolaei, A.R., Mohammadi, R., Badali, H., Moazeni, M., Haghani, I., Ghojoghi, A. and Akhtari, J. (2016). Genetic and morphological diversity of the genus Penicillium from Mazandaran and Tehran Provinces. Jundishapur Microbial., 9.(1) 1-7.
  • Asan, A. (2000). Check list of Aspergillus and Penicillium species reported from Turkey. Turkish Journal of Botany, 24(3), 151-168.
  • Ahmet, A. (2004). Aspergillus, Penicillium and related species reported from Turkey. Mycotaxon, 89(1), 155-157.
  • Berbee, M. L., Yoshimura, A., Sugiyama, J., & Taylor, J. W. (1995). Is Penicillium monophyletic? An evaluation of phylogeny in the family Trichocomaceae from 18S, 5.8 S and ITS ribosomal DNA sequence data. Mycologia, 87(2), 210-222.
  • Çakır, E., &Maden, S. (2015). First report of Penicilliumpolonicum causing storage rots of onion bulbs in Ankara province, Turkey. New Disease Reports, 32(24), 2044-0588.
  • Deng, J. X., Paul, N. C., Sang, H. K., Lee, J. H., Hwang, Y. S., & Yu, S. H. (2012). First Report on Isolation of Penicilliumadametzioides and Purpureocilliumlilacinum from Decayed Fruit of Cheongsoo Grapes in Korea. Mycobiology, 40(1), 66–70.
  • Hasenekoğlu İ.(1991) “ToprakMikrofungusları, Cilt V”, Kazım Karabekir Eğitim Fakültesi Yayınları No:11, Atatürk Üniversitesiyayınları No:689, 32.
  • Hassan SE (2017) Plant growth-promoting activities for bacterial and fungal endophytes isolated from medicinal plant of Teucrium polium L. J Adv Res 8(6), 687–695.
  • Kim, C. S., Park, M. S., & Yu, S. H. (2008). Two species of endophytic Penicillium from Pinus rigida in Korea. Mycobiology, 36(4), 222-227.
  • Kolanlarli, T. K., Ahmet, A. S. A. N., Burhan, Ş. E. N., & ÖKTEN, S. (2019). Biodiversity of Penicillium species isolated from Edirne Söğütlük Forest soil (Turkey). MantarDergisi, 10(1), 26-39.
  • Nicoletti R, Fiorentino A, Scognamiglio M (2014) Endophytism of Penicillium species in woody plants. Open Mycol Journal 8:1–26.
  • Paterson, R. R. M., Kozakiewicz, Z., Locke, T., Brayford, D., & Jones, S. C. B. (2003). Novel use of the isoepoxydon dehydrogenase gene probe of the patulin metabolic pathway and chromatography to test penicillia isolated from apple production systems for the potential to contaminate apple juice with patulin. Food microbiology, 20(3), 359-364.
  • Peterson, S. W. (2000). In R. A. Samson & J. I. Pitt (Eds.), Integration of modern taxonomic methods for penicillium and aspergillus classification (pp. 163–178). The Netherlands: Hardwood academic.
  • Peterson, S. W. (2000). Phylogenetic analysis of Penicillium species based on ITS and LSU-rDNA nucleotide sequences. Integration of modern taxonomic methods for Penicillium and Aspergillus classification, 163-178. The Netherlands: Harwood Academic
  • Peterson, S. W.; Bayer, E. M.; Wicklow, D. T. (2004). "Penicillium thiersii, Penicillium angulare and Penicillium decaturense, new species isolated from wood-decay fungi in North America and their phylogenetic placement from multilocus DNA sequence analysis". Mycologia. 96(6),93-1280.
  • Pitt, J. I. (1979) .The genus Penicillium and its teleomorphic states Eupenicillium and Talaromyces (Academic Press,).
  • Ertuğrul,S., Ahmet,A., Selçuk,F. (2020). Türkiye Mantarları Listesi,1-1177. İstanbul:Ali Nihat GökyiğitVakfı.
  • Korcan, S. E., Kahraman T., Acikbas, Y., Liman, R., Ciğerci, İ.H., Konuk, M., Ocak, İ. (2012). Cyto–genotoxicity, antibacterial, and antibiofilm properties of green synthesized silver nanoparticles using Penicillium toxicarium. Microscopy Research technique. https://doi.org/10.1002/jemt.23802
  • Singh, D., Rathod, V., Ninganagouda, S., Hiremath, J., Singh, A. K., & Mathew, J. (2014). Optimization and characterization of silver nanoparticle by endophytic fungi Penicillium sp. isolated from Curcuma longa (turmeric) and application studies against MDR E. coli and S. aureus. Bioinorganic chemistry and applications.
  • Skouboe, P., Boysen, M., Pedersen, L. H., Frisvad, J. C., &Rossen, L. (1996). Identification of Penicillium species using the internal transcribed spacer (ITS) region. In Fungal identification techniques ,160-164. European Commission.
  • Skouboe, P., Frisvad, J. C., Taylor, J. W., Lauritsen, D., Boysen, M., &Rossen, L. (1999). Phylogenetic analysis of nucleotide sequences from the ITS region of terverticillatePenicillium species. Mycological Research, 103(7), 873-881.
  • Skouboe, P., Taylor, J. W., Frisvad, J. C., Lauritsen, D., Larsen, L., Alboek, C., &Rossen, L. (2000). Molecular methods for differentiation of closely related Penicillium species. Integration of modern taxonomic methods for Penicillium and Aspergillus classification, 171-180.
  • Toghueo, R. M. K., & Boyom, F. F. (2020). Endophytic Penicillium species and their agricultural, biotechnological, and pharmaceutical applications. 3 Biotech, 10(3), 1-35.
  • Visagie, C. M., Houbraken, J., Frisvad, J. C., Hong, S. B., Klaassen, C. H. W., Perrone, G., & Samson, R. A. (2014). Identification and nomenclature of the genus Penicillium. Studies in mycology, 78, 343-371.
  • Visagie, C.M., Houbraken, J., Frisvard, J.C., Hong, S.B., Klaassen, C.H.W., Perrone, G., Seifert, K.A., Varga, J., Yaguchi, T. and Samson, R.A. (2014). Identification and nomenclature of the genus Penicillium. Studies in mycology ,78, 343-371.
  • Waqas M, Khan AL, Hamayun M, Shahzad R, Kang SM, Kim JG et al (2015). Endophytic fungi promote plant growth and mitigate the adverse effects of stem rot: an example of Penicillium citrinum and Aspergillus terreus. J Plant Interact 10, 280–287.
  • White, T.J., Bruns, T.D., Lee, S.B. and Taylor, J.W. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky. 15, 315-322.
  • https://eurx.com.pl/docs/manuals/en/e3595.pdf.
  • https://www.thermofisher.com/documentconnect/documentconnect.html?url=https://assets.thermofisher.com/TFSAssets/LSG/manuals/78200b.pdf&title=Brief%20Protocol:%20ExoSAP-IT%20PCR%20Product%20Cleanup%20Brief%20Protocol .

Penicillium toxicarium'un İzolasyonu Ve İdentifikasyonu: Türkiye İçin Yeni Kayıt

Year 2022, Volume: 13 Issue: 1, 44 - 54, 28.04.2022

Safiye Elif Korcan Tuğba Kahraman İjlal Ocak Ahmed Badri Abed Büşra Aydın

Abstract

Bu çalışmada Penicillium toxicarium Türkiye'de ilk kez yeni bir Penicillium türü olarak gösterildi. Bu tür, Türkiye'nin Uşak ilinde çürüyen ağaç örneklerinden tespit edilmiştir. Makroskobik ve mikroskobik morfolojiksine göreve moleküler tani yöntemlerine gore tanımlama yapıldı. Fungal DNA İzolasyon Kiti ve evrensel primerler kullanılarak ITS bölgsi dizi analizleri moleküler tanımlama için kullanıldı.

Keywords

Penicillium toxicarium Penicillium Türkiye Mantarlar Mikobiyota Kayıt

References

  • Abastabar, M., Mirhendi, H., Hedayati, M.T., Shokohi, T., Matehkolaei, A.R., Mohammadi, R., Badali, H., Moazeni, M., Haghani, I., Ghojoghi, A. and Akhtari, J. (2016). Genetic and morphological diversity of the genus Penicillium from Mazandaran and Tehran Provinces. Jundishapur Microbial., 9.(1) 1-7.
  • Asan, A. (2000). Check list of Aspergillus and Penicillium species reported from Turkey. Turkish Journal of Botany, 24(3), 151-168.
  • Ahmet, A. (2004). Aspergillus, Penicillium and related species reported from Turkey. Mycotaxon, 89(1), 155-157.
  • Berbee, M. L., Yoshimura, A., Sugiyama, J., & Taylor, J. W. (1995). Is Penicillium monophyletic? An evaluation of phylogeny in the family Trichocomaceae from 18S, 5.8 S and ITS ribosomal DNA sequence data. Mycologia, 87(2), 210-222.
  • Çakır, E., &Maden, S. (2015). First report of Penicilliumpolonicum causing storage rots of onion bulbs in Ankara province, Turkey. New Disease Reports, 32(24), 2044-0588.
  • Deng, J. X., Paul, N. C., Sang, H. K., Lee, J. H., Hwang, Y. S., & Yu, S. H. (2012). First Report on Isolation of Penicilliumadametzioides and Purpureocilliumlilacinum from Decayed Fruit of Cheongsoo Grapes in Korea. Mycobiology, 40(1), 66–70.
  • Hasenekoğlu İ.(1991) “ToprakMikrofungusları, Cilt V”, Kazım Karabekir Eğitim Fakültesi Yayınları No:11, Atatürk Üniversitesiyayınları No:689, 32.
  • Hassan SE (2017) Plant growth-promoting activities for bacterial and fungal endophytes isolated from medicinal plant of Teucrium polium L. J Adv Res 8(6), 687–695.
  • Kim, C. S., Park, M. S., & Yu, S. H. (2008). Two species of endophytic Penicillium from Pinus rigida in Korea. Mycobiology, 36(4), 222-227.
  • Kolanlarli, T. K., Ahmet, A. S. A. N., Burhan, Ş. E. N., & ÖKTEN, S. (2019). Biodiversity of Penicillium species isolated from Edirne Söğütlük Forest soil (Turkey). MantarDergisi, 10(1), 26-39.
  • Nicoletti R, Fiorentino A, Scognamiglio M (2014) Endophytism of Penicillium species in woody plants. Open Mycol Journal 8:1–26.
  • Paterson, R. R. M., Kozakiewicz, Z., Locke, T., Brayford, D., & Jones, S. C. B. (2003). Novel use of the isoepoxydon dehydrogenase gene probe of the patulin metabolic pathway and chromatography to test penicillia isolated from apple production systems for the potential to contaminate apple juice with patulin. Food microbiology, 20(3), 359-364.
  • Peterson, S. W. (2000). In R. A. Samson & J. I. Pitt (Eds.), Integration of modern taxonomic methods for penicillium and aspergillus classification (pp. 163–178). The Netherlands: Hardwood academic.
  • Peterson, S. W. (2000). Phylogenetic analysis of Penicillium species based on ITS and LSU-rDNA nucleotide sequences. Integration of modern taxonomic methods for Penicillium and Aspergillus classification, 163-178. The Netherlands: Harwood Academic
  • Peterson, S. W.; Bayer, E. M.; Wicklow, D. T. (2004). "Penicillium thiersii, Penicillium angulare and Penicillium decaturense, new species isolated from wood-decay fungi in North America and their phylogenetic placement from multilocus DNA sequence analysis". Mycologia. 96(6),93-1280.
  • Pitt, J. I. (1979) .The genus Penicillium and its teleomorphic states Eupenicillium and Talaromyces (Academic Press,).
  • Ertuğrul,S., Ahmet,A., Selçuk,F. (2020). Türkiye Mantarları Listesi,1-1177. İstanbul:Ali Nihat GökyiğitVakfı.
  • Korcan, S. E., Kahraman T., Acikbas, Y., Liman, R., Ciğerci, İ.H., Konuk, M., Ocak, İ. (2012). Cyto–genotoxicity, antibacterial, and antibiofilm properties of green synthesized silver nanoparticles using Penicillium toxicarium. Microscopy Research technique. https://doi.org/10.1002/jemt.23802
  • Singh, D., Rathod, V., Ninganagouda, S., Hiremath, J., Singh, A. K., & Mathew, J. (2014). Optimization and characterization of silver nanoparticle by endophytic fungi Penicillium sp. isolated from Curcuma longa (turmeric) and application studies against MDR E. coli and S. aureus. Bioinorganic chemistry and applications.
  • Skouboe, P., Boysen, M., Pedersen, L. H., Frisvad, J. C., &Rossen, L. (1996). Identification of Penicillium species using the internal transcribed spacer (ITS) region. In Fungal identification techniques ,160-164. European Commission.
  • Skouboe, P., Frisvad, J. C., Taylor, J. W., Lauritsen, D., Boysen, M., &Rossen, L. (1999). Phylogenetic analysis of nucleotide sequences from the ITS region of terverticillatePenicillium species. Mycological Research, 103(7), 873-881.
  • Skouboe, P., Taylor, J. W., Frisvad, J. C., Lauritsen, D., Larsen, L., Alboek, C., &Rossen, L. (2000). Molecular methods for differentiation of closely related Penicillium species. Integration of modern taxonomic methods for Penicillium and Aspergillus classification, 171-180.
  • Toghueo, R. M. K., & Boyom, F. F. (2020). Endophytic Penicillium species and their agricultural, biotechnological, and pharmaceutical applications. 3 Biotech, 10(3), 1-35.
  • Visagie, C. M., Houbraken, J., Frisvad, J. C., Hong, S. B., Klaassen, C. H. W., Perrone, G., & Samson, R. A. (2014). Identification and nomenclature of the genus Penicillium. Studies in mycology, 78, 343-371.
  • Visagie, C.M., Houbraken, J., Frisvard, J.C., Hong, S.B., Klaassen, C.H.W., Perrone, G., Seifert, K.A., Varga, J., Yaguchi, T. and Samson, R.A. (2014). Identification and nomenclature of the genus Penicillium. Studies in mycology ,78, 343-371.
  • Waqas M, Khan AL, Hamayun M, Shahzad R, Kang SM, Kim JG et al (2015). Endophytic fungi promote plant growth and mitigate the adverse effects of stem rot: an example of Penicillium citrinum and Aspergillus terreus. J Plant Interact 10, 280–287.
  • White, T.J., Bruns, T.D., Lee, S.B. and Taylor, J.W. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky. 15, 315-322.
  • https://eurx.com.pl/docs/manuals/en/e3595.pdf.
  • https://www.thermofisher.com/documentconnect/documentconnect.html?url=https://assets.thermofisher.com/TFSAssets/LSG/manuals/78200b.pdf&title=Brief%20Protocol:%20ExoSAP-IT%20PCR%20Product%20Cleanup%20Brief%20Protocol .
There are 29 citations in total.

Details

Primary Language English
Journal Section RESEARCH ARTICLE
Authors

Safiye Elif Korcan 0000-0001-7875-5516

Tuğba Kahraman 0000-0002-6655-4423

İjlal Ocak 0000-0001-6976-5747

Ahmed Badri Abed 0000-0002-7803-7917

Büşra Aydın 0000-0002-1199-5600

Publication Date April 28, 2022
Published in Issue Year 2022 Volume: 13 Issue: 1

Cite

APA Korcan, S. E., Kahraman, T., Ocak, İ., Abed, A. B., et al. (2022). Isolation and Identification of Penicillium toxicarium: A New Record for Turkey. Mantar Dergisi, 13(1), 44-54. https://doi.org/10.30708/mantar.933290
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